Journal: Journal of Fungi
Article Title: Differential Physiological Prerequisites and Gene Expression Profiles of Conidial Anastomosis Tube and Germ Tube Formation in Colletotrichum gloeosporioides
doi: 10.3390/jof7070509
Figure Lengend Snippet: Germ tube formation and conidial anastomosis tube fusion dynamics in C. gloeosporioides . ( A ) Percentage GT/CAT formation in different aged conidia, viz., 6, 10, 13, and 17 days of C. gloeosporioides . ( B ) Percentage CAT fusion in 17 days old conidia of C. gloeosporioides incubated for different incubation times, viz., 24, 48, 72, and 96 h. ( C ) Percentage GT formation in 6 days old conidia of C. gloeosporioides incubated for different incubation times, viz., 3, 6, 12, 18, and 24 h. ( D ) Percentage CAT fusion in different conidial densities, viz., 4 × 10 2 , 4 × 10 3 , 4 × 10 4 , 4 × 10 5 , and 4 × 10 6 of 17-day-old conidia incubated in 1 mL of water. ( E ) Percentage GT formation in different conidial densities, viz., 4 × 10 2 , 4 × 10 3 , 4 × 10 4 , 4 × 10 5 , and 4 × 10 6 of 6-day-old conidia incubated in 1 mL of PDB. ( F ) CAT and GT formation percentage in PDB, water, glucose, and Insolution ™ PD98059 (inhibitor of MEK), CAT fusion induced in water supplemented with glucose and Insolution ™ PD98059; GT formation was assayed in PDB supplemented with glucose and Insolution ™ PD98059. GT/CAT formation was quantified as the percentage of conidia involved in GT/CAT formation. Average from three replicates ( n = 3), and 300 conidial numbers were counted per replicate. Bar indicates standard deviation. Statistical significance of differences was analyzed by one-way ANOVA with Tukey’s multiple comparison post-hoc test (bars with the same letter are not significantly different; p ≤ 0.05).
Article Snippet: The GT formation and CAT fusion data were analyzed by Analysis of Variance (ANOVA) with Tukey’s post hoc test using GraphPad Prism 5 Statistics Software.
Techniques: Incubation, Standard Deviation, Comparison
